Regulatory
icd

Part:BBa_K4232003

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-09-14)


A promoter sensitive to oxygen consumption rate.

This part of the code is the promoter icd. The ICD promoter, known as isocitrate dehydrogenase, is derived from the E. coli top10 strain. It can be used as a sensor for the rate of oxygen consumption in the cell body (the mechanism of action is shown in Figure 1).

The icd contains two functional modules - cra and arca. cra and arca partially overlap and it is mainly the arca module that performs the oxygen consumption rate detection function, so if you want to use the icd as an oxygen consumption rate sensor you should deactivate the cra module as much as possible (as shown in figure 2 below).

Figure 1 The mechanism about icd
Figure 2 How to use icd as an Oxygen sensitive promoter











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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